TC31細胞
運輸方式： 凍存運輸
是否是腫瘤細胞： 0
物種來源： 小鼠
生長狀態(tài)： 懸浮生長
數(shù)量： 大量
ATCC Number： CRL-2494?
細胞形態(tài)： **樣
**類型： IgM kappa
TC31細胞Designations: TC31
Depositors: DG Sieckmann
Isotype: IgM kappa
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybrid
Permits/Forms: TC31細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: This cell line was deposited at the ATCC by Donna G. Sieckmann, Naval Medical Research Center. The line is distributed for academic research purposes only. The Naval Medical Research Center releases the line subject to the following: 1.) The line or its products must not be distributed to third parties. Commercial interests are the exclusive property of the Naval Medical Research Center. 2.) Any proposed commercial use of the cell line including any use by a for-profit entity must first be negotiated with the Commanding Officer, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910-7500.
Isolation: Isolation date: 1984
Comments: Animals were immunized against exotoxin A of Pseudomonas aeruginosa by infection with strain PA220.
Spleen cells were fused with Sp2/0-Ag14 cells.
The antibody binds specifically for the exotoxin A of Pseudomonas aeruginosa.
TC31細胞It displays specificity for exotoxin A from strain PA103 and strain PAO1 and PAO-PR1, a derivative of PAO1. [40007]
The antibody shows high specificity and reactivity for native and denatured exotoxin A in ELISA assays.
Both TC1 (ATCC CRL-2493) and TC31 (ATCC CRL-2485).ATCC CRL-2494) antibodies neutralize exotoxin A toxicity against CHO cells in an in vitro assay but only TC31 protected mice infected with strain PA220 in an in vivo model. [40007]
A hybridoma producing an anti idiotypic antibody against TC31 is available (
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(4) viable cells/ml. Do not allow the cell concentration to exceed 5 X 10(5) viable cells/ml.
Interval: Maintain cultures at a cell concentration between 5 X 10(4) and 5 X 10(5) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: TC31細胞Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
References: 40007: Galloway DR, et al. Production and characterization of monoclonal antibodies to exotoxin A from Pseudomonas aeruginosa. Infect. Immun. 44: 262-267, 1984. PubMed: 6425217
