生長狀態(tài)： 貼壁生長

ATCC Number： HTB-126?

相關(guān)**： 腫瘤

年限： 74 years *****

是否是腫瘤細胞： 0

物種來源： 人

器官來源： **

細胞形態(tài)： 上皮樣

運輸方式： 凍存運輸

數(shù)量： 大量

Designations: Hs 578T

Hs 578T細胞Depositors: AJ Hackett

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: mammary gland; breast

Disease: carcinoma

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Receptors: estrogen receptor, not expressed [1119]

Tumorigenic: No

DNA Profile (STR): Hs 578T細胞Amelogenin: X

CSF1PO: 13

D13S317: 11

D16S539: 9,12

D5S818: 11

D7S820: 10

THO1: 9,9.3

TPOX: 8

vWA: 17

Cytogenetic Analysis: Number of cells examined = 50; Modal Chromosome Number = 59 with a range of 50 to 77; Polyploidy Rate = 33.8%

Composite karyotype: 50-77 <3n> X, -1, del(1)(q12), -2, del(2)(?q36), der(3)t(3;15)(q10;p10),-4, -5,der(5)t(5;8)(p10;q10),-6, i(6)(p10), +8, -9, -10, -11, del(11)(p12), -12, -13, -14, -15, -15, -16, -17, -17, -17, i(17)(q10), -18, -19,der(19)(19pter<-q13::5q13<-qter), +22, +3 mar[cp12]

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1

Me-2, 0

PGM1, 1

PGM3, 1

Age: 74 years *****

Gender: female

Ethnicity: Caucasian

Comments: The Hs 578T cell strain was derived from a carcinoma of the breast.

Hs 578T細胞It was originated by A.J. Hackett, et al. along with the Hs 578Bst (see ATCC HTB-125), which is a normal fibroblast-like line from the same patient.

The Hs 578T line had a mixed polygonal morphology initially, but a stellate cell type was selected for during passage and by cloning.

Aggregates of casein protein granules, desmosomes, tight junctions, lipid droplets and vesicularized smooth endoplasmic reticulum were observed by electron microscopy.

As with Hs 578Bst, no estrogen receptors or endogenous viruses were detected.

Cytogenetics: Derivatives and Markers

There are 8 consistent derivative chromosomes: del(1)(q12), del(2)(?q36), der(3)t(3;15)(q10;p10), der(5)t(5;8)(p10;q10), i(6)(p10), del(11)(p12), i(17)(q10), der(19)(19pter<-q13::5q13<-qter) plus two markers of unknown origin and one minute chromosome.

Four other markers, including two derivative chromosome 1s were noted are lower frequency.

Cytogenetics: Comments

This is a hypotriploid human cell line with a modal chromosome number of 59.

QM staining verified the absence of a Y chromosome.

The rate of polyploidy in excess of the modal number is 33.8%.

There were 8 consistent derivative chromosomes: del(1)(q12), del(2)(?q36), der(3)t(3;15)(q10;p10),der(5)t(5;8)(p10;q10), i(6)(p10), del(11)(p12),i(17)(q10),

der(19)(19pter<-q13::5q13<-qter) plus two markers of unknown origin and one minute chromosome.

Normal chromosome 17's were absent and only a single normal 15 was seen in most cells.

No brightly fluorescent Y chromosomes were detected with QM staining.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 0.01 mg/ml bovine insulin; fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Hs 578T細胞Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

normal (or near-normal) cell line established from the same patient:ATCC HTB-125

purified DNA:ATCC HTB-126D

purified RNA:ATCC HTB-126R

References: 1119: Hackett AJ, et al. Two syngeneic cell lines from human breast tissue: the aneuploid mammary epithelial (Hs 578T) and the diploid myoepithelial (Hs 578Bst) cell lines. J. Natl. Cancer Inst. 58: 1795-1806, 1977. PubMed: 864756

22543: Smith HS.Hs 578T細胞 In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. PubMed: 283258

32275: Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764