是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
細(xì)胞形態(tài)： **樣
**類型： IgG1
運(yùn)輸方式： 凍存運(yùn)輸
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
ATCC Number： HB-208?
數(shù)量： 大量
Designations: TE7
Depositors: BF Haynes
Isotype: IgG1
Biosafety Level: 1
TE7細(xì)胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human thymus
Permits/Forms: In addition to the MTA mentioned above, TE7細(xì)胞other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with human thymic stroma.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with cells in the thymic capsule and interlobular septae and vessels.
It also reacts with non-endocrine fibrous stroma in the thymus, and with mesodermally derived connective tissue in other parts of the body.
It defines the mesodermal component of the human thymic microenvironment that most likely induces thymic epithelial differentiation early in the development of the thymus.
TE7細(xì)胞Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%; heat-inactivated fetal bovine serum, 20%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1638: Haynes BF, et al. TE7細(xì)胞Phenotypic characterization and ontogeny of mesodermal-derived and endocrine epithelial components of the human thymic microenvironment. J. Exp. Med. 159: 1149-1168, 1984. PubMed: 6200562
