LCL 8664細(xì)胞
細(xì)胞類型： B**細(xì)胞
ATCC Number： CRL-1805?
相關(guān)**： **瘤
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 獼猴
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
運(yùn)輸方式： 凍存運(yùn)輸
年限： 5 years
數(shù)量： 大量
LCL 8664細(xì)胞細(xì)胞形態(tài)： **樣
Designations: LCL 8664
Depositors: SR Rangan
Biosafety Level: 2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Macaca mulatta
Morphology: lymphoblast
Source: Disease: lymphoma
Cell Type: LCL 8664細(xì)胞B lymphocyte;
Cellular Products: B lymphotropic herpesvirus
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 5 years
Gender: male
Comments: CAUTION: the cells are infected with and produce a B lymphotropic herpesvirus (RhEBV) and may represent a potential biohazard.
Free RhEBV is present in the supernatant, and rhesus B lymphocyte transforming activity is present in the medium.
About 1% of the cells express virus capsid antigen, and all express the virus related nuclear antigen.
Propagation: LCL 8664細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 10 exp5 cells/ml and maintain between 4 X 10 exp5 and 2 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22860: Rangan SR, et al. Epstein-Barr virus-related herpesvirus from a rhesus monkey (Macaca mulatta) with malignant lymphoma. Int. J. Cancer 38: 425-432, 1986.LCL 8664細(xì)胞 PubMed: 3017870
