18C2.8.3細胞

數(shù)量： 大量

ATCC Number： HB-9571?

運輸方式： 凍存運輸

生長狀態(tài)： 懸浮生長

細胞形態(tài)： **樣

是否是腫瘤細胞： 0

物種來源： 小鼠

Designations: 18C2.8.3

18C2.8.3細胞Depositors: Univ. Georgia Res. Fndn.

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: 18C2.8.3細胞hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against NK cell target ligand on NC-37 cells

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Tumorigenic: Yes

Comments: Animals were immunized with NC-37 (see ATCC CCL-214) cells.

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

18C2.8.3細胞The antibody reacts with NC-37 cells and inhibits NK cell mediated lysis.

The antibody also blocks lysis by catfish nonspecific cytotoxic cells (NCC) which are analogous to mammalian NK cells.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22020: Evans DL. 18C2.8.3細胞Antibodies to natural killer cell and non-specific cytotoxic cell receptor and target cell antigens. US Patent 5,194,593 dated Mar 16 1993