As4.1細(xì)胞

ATCC Number： CRL-2193?

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

生長狀態(tài)： 貼壁生長

器官來源： 腎臟

細(xì)胞形態(tài)： 上皮樣

數(shù)量： 大量

組織來源： intraparenchymal

Designations: As4.1

As4.1細(xì)胞Depositors: KW Gross

Biosafety Level: 2 [CELLS CONTAIN PAPOVAVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: epithelial

Source: Organ: kidney

Tissue: intraparenchymal

Cellular Products: renin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. As4.1細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host (Roche Transfection Reagents)

Gender: female

Comments: As4.1 was established from the ascites fluid of a six month old transgenic mouse harboring an intraparenchymal kidney tumor induced by transgene targeted tumorigenesis.

These transgenic mice were constructed with the renin T antigen fusion gene, pR2d4.6TAG which contains a transcriptional fusion between Ren-2d 5' flanking sequences and the structural gene for SV40 T antigen.

The cell line was cloned by picking a small cluster from a dish seeded at low density.

The As4.1 cells express high levels of correctly processed renin mRNA from the endogenous Ren-1c locus.

They constitutively secrete prorenin and appear to store a proteolytically cleaved form of active renin intracellularly in storage granules/vesicles.

It has been reported that each As4.1 cell contains 1000 to 2000 copies of renin mRNA.

Propagation: As4.1細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Add fresh trypsin solution (1 to 2 ml), and let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:12 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 22375: Jones CA, et al. Expression of murine renin genes during fetal development. Mol. Endocrinol. 4: 375-383, 1990. PubMed: 2188116

22691: Sigmund CD, et al. As4.1細(xì)胞Tissue and cell specific expression of a renin promoter-reporter gene construct in transgenic mice. Biochem. Biophys. Res. Commun. 170: 344-350, 1990. PubMed: 1695507

23550: Sigmund CD, et al. Isolation and characterization of renin-expressing cell lines from transgenic mice containing a renin-promoter viral oncogene fusion construct. J. Biol. Chem. 265: 19916-19922, 1990. PubMed: 2174057

33130: Petrovic N, et al. Role of proximal promoter elements in regulation of renin gene transcription. J. Biol. Chem. 271: 22499-22505, 1996. PubMed: 8798416