1G3細(xì)胞

生長(zhǎng)狀態(tài)： 混合型生長(zhǎng)

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： **樣

**類(lèi)型： IgG1

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

ATCC Number： CRL-2434?

Designations: 1G3

Depositors: PJ Bjorkman

1G3細(xì)胞Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: mixed: adherent and suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against FcRn heavy chain heterodimers

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Comments: The cell line was established by Louis Gastinel and Pamela Bjorkman in 1992.

Animals were immunized with and twice boosted with soluble neonatal Fc receptor (FcRn) in an adjuvant. Three days before fusion one mouse was boosted with purified FcRn.

Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase [HPRT] and adenine phosphoribosyl transferase [APRT]).

The antibody recognizes FcRn heavy chain heterodimers.

FcRn is a heterodimer composed of a membrane bound heavy chain attached noncovalently to beta 2-microgloublin. It is structurally similar to class I major histocompatibility (MHC) molecules.

The IG3 (CRL-2434) antibody recognizes two lipid linked forms of FcRn.

These forms are truncated FcRn heavy chain paired with beta 2-microgloublin-DAF (FcRn beta-2 microglobulin DAF) and lipid-linked FcRn paired with unaltered beta 2-microgloublin 1G3細(xì)胞(FcRn-DAF/beta 2-microgloublin).

It also recognizes the FcRn-DAF construct.

The 2G3 (CRL-2435) antibody fails to recognize the FcRn heterodimer form in which the heavy chain is attached to the lipid anchor (FcRn-DAF/beta 2-microgloublin) and it also fails to recognize the FcRn-DAF construct.

Unlike 4C9 (CRL-2437), the monoclonal antibodies IG3 (CRL-2434)and 2G3 (CRL-2435) do not recognize rat beta 2-microglobulin alone or complexed with FcRn heavy chains.

This antibody is used in studies of the MHC class I heavy chain FcRn heterodimers and their interaction with IgG. [39787]

Propagation: ATCC complete growth medium: HL-1 medium supplemented with 4 mM L-glutamine, 1 mM sodium pyruvate and 1% fetal bovine serum. HL-1 medium can be obtained from from Lonza (catalog number 77201).

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:5 every 3 - 4 day is recommended

Medium Renewal: 1G3細(xì)胞Add fresh medium every 2 to 3 days (depending on cell density)

Subcultures are prepared by scraping. Dislodge cells from the flask substrate with a cell scraper. Aspirate gently and dispense into new flasks. Seed flasks at 2 X 10exp5 viable cells/ml.

Preservation: HL-1 Complete Serum-free Medium, 75%; fetal bovine serum, 20%; DMSO, 5%.

Related Products: recommended serum:ATCC 30-2020

References: 39787: Raghavan M, et al. Investigation of the interaction between the class I MHC-related Fc receptor and its immunoglobulin G ligand. Immunity 1: 303-315, 1994. PubMed: 7889418