Lec1細(xì)胞

ATCC Number： CRL-1735?

生長狀態(tài)： 貼壁生長

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： 上皮樣

是否是腫瘤細(xì)胞： 0

物種來源： 倉鼠

數(shù)量： 大量

器官來源： 卵巢

Lec1細(xì)胞Designations: Lec1 [originally named Pro-5WgaRI3C]

Depositors: P Stanley

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: loosely adherent

Organism: Cricetulus griseus

Morphology: epithelial

Source: Organ: ovary

Permits/Forms: Lec1細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host

Gender: female

Comments: This line is a mutant clone derived from the parental CHO clone Pro-5 (a proline auxotroph, see ATCC CRL-1781) by selection for resistance to wheat germ agglutinin. The cells lack GlcNAc glycosyl transferase so that N-linked carbohydrates are blocked at the Man5-GlcNAC2-Asn intermediate. The line may be useful for studying the effects of altered N-linked glycosylation on the function and compartmentalization of endogenous glycoproteins or glycoproteins introduced by viral infection or transfection of foreign DNA.

BR>The population contains Pro+ revertants at low frequency (approximately 1 in 100,000 cells), and should be suitable for complementation studies which require the Pro- marker.

Lec1細(xì)胞The cells are proline auxotrophs and must be grown in a medium that contains proline (40 mg/L).

The population contains Pro+ revertants at low frequency (approximately 1 in 100000 cells), and should be suitable for complementation studies which require the Pro- marker.

Propagation: ATCC complete growth medium: Alpha minimum essential medium with ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: recommended serum:ATCC 30-2020

References: 1201: Stanley P, et al. Lec1細(xì)胞Selection and characterization of eight phenotypically distinct lines of lectin-resistant Chinese hamster ovary cells. Cell 6: 121-128, 1975. PubMed: 1182798

23255: Stanley P, et al. Chinese hamster ovary cells selected for resistance to the cytotoxicity of phytohemagglutinin are deficient in a UDP-N-acetylglucosamine-- glycoprotein N-acetylglucosaminyltransferase activity. Proc. Natl. Acad. Sci. USA 72: 3323-3327, 1975. PubMed: 1059116