1939-3G5細胞

運輸方式： 凍存運輸

細胞形態(tài)： **樣

**類型： IgM

數(shù)量： 大量

生長狀態(tài)： 懸浮生長

是否是腫瘤細胞： 0

物種來源： 小鼠

ATCC Number： HB-8133?

Designations: 1939-3G5

1939-3G5細胞Depositors: Univ. Texas System, Board of Regents

Isotype: IgM

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against Treponema pallidum

Permits/Forms: 1939-3G5細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with T. pallidum.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody does not react with T. phagedenis.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. 1939-3G5細胞To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 10%

1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X

References: 3539: Kettman JR, Norgard MV. 1939-3G5細胞Hybrid cell lines producing monoclonal antibodies directed against Treponema. US Patent 4,514,498 dated Apr 30 1985