Lec2細(xì)胞

細(xì)胞形態(tài)： 上皮樣

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CRL-1736?

是否是腫瘤細(xì)胞： 0

物種來源： 倉鼠

數(shù)量： 大量

器官來源： 卵巢

生長狀態(tài)： 單層（少量懸浮）

Lec2細(xì)胞Designations: Lec2 [originally named Pro-5WgaRII6A]

Depositors: P Stanley

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: monolayer or suspension

Organism: Cricetulus griseus

Morphology: epithelial

Source: Organ: ovary

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Gender: female

Comments: This line is a mutant clone derived from the parental CHO clone Pro-5 (a proline auxotroph, see ATCC CRL-1781) by selection for resistance to wheat germ agglutinin.

The cells exhibit a drastic reduction in the transport of CMP-sialic acid into the Golgi compartment, and may be useful for studying the role of sialic acid in the function and compartmentalization of glycosylated macromolecules.

The cells are proline auxotrophs and must be grown in a medium that contains proline (40 mg/L).

The population contains Pro+ revertants at high frequency (approximately 1 in 25 cells), and should be cloned if it is desired to use the Pro- marker in complementation studies.

Propagation: Lec2細(xì)胞ATCC complete growth medium: Alpha minimum essential medium, 90%; fetal bovine serum, 10%

Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, let sit for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes or until the cells detach. Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

References: 22626: Deutscher SL, et al. Translocation across Golgi vesicle membranes: a CHO glycosylation mutant deficient in CMP-sialic acid transport. Cell 39: 295-299, 1984. PubMed: 6498937

58070: Stanley P, Siminovitch L. Complementation between mutants of CHO cells resistant to a variety of plant lectins. Somatic Cell Genet. 3: 391-405, 1977. Lec2細(xì)胞PubMed: 601679