1939-8G2細胞

是否是腫瘤細胞： 0

物種來源： 小鼠

ATCC Number： HB-8134?

運輸方式： 凍存運輸

數(shù)量： 大量

生長狀態(tài)： 懸浮生長

細胞形態(tài)： **樣

1939-8G2細胞**類型： IgG1

Designations: 1939-8G2

Depositors: Univ. Texas System, Board of Regents

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products:1939-8G2細胞 immunoglobulin; monoclonal antibody; against Treponema pallidum

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Comments: Animals were immunized with T. pallidum.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody does not react with T. phagedenis.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. 1939-8G2細胞To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 10%

1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 3539: Kettman JR, Norgard MV. 1939-8G2細胞Hybrid cell lines producing monoclonal antibodies directed against Treponema. US Patent 4,514,498 dated Apr 30 1985