TE19細胞

生長狀態(tài)： 懸浮生長

細胞形態(tài)： **樣

**類型： IgM

運輸方式： 凍存運輸

是否是腫瘤細胞： 0

物種來源： 小鼠

ATCC Number： HB-211?

TE19細胞數(shù)量： 大量

Designations: TE19

Depositors: BF Haynes

Isotype: IgM

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: TE19細胞lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human thymic epithelium (Hassall's bodies)

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Comments: Animals were immunized with human thymic stroma.

Spleen cells were fused with NS-1 myeloma cells.

TE19細胞The antibody reacts with a granular pattern of cells within the cellular portion of Hassall's bodies.

It defines an antigen that is acquired between 14 and 16 weeks of fetal gestation, and marks the terminal stages of epidermal keratinocyte maturation.

The antibody binds specifically to cells within the cellular portion of Hassall's bodies and also to stratum granulosum of skin epidermis on individuals with the A blood group antigen.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%; heat-inactivated fetal bovine serum, 20%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1637: Lobach DF, et al. TE19細胞The human thymic microenvironment. Phenotypic characterization of Hassall's bodies with the use of monoclonal antibodies. J. Immunol. 134: 250-257, 1985. PubMed: 2578044