1D11.16.8細胞
ATCC Number： HB-9849?
細胞形態(tài)： **樣
**類型： IgG1 , IgG1
生長狀態(tài)： 懸浮生長
運輸方式： 凍存運輸
是否是腫瘤細胞： 0
物種來源： 小鼠
數(shù)量： 大量
Designations: 1D11.16.8
1D11.16.8細胞Depositors: Celtrix Pharmaceuticals, Inc.
Isotype: IgG1 , IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: 1D11.16.8細胞immunoglobulin; monoclonal antibody; against transforming growth factor beta2 (TGF-beta2)
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Tumorigenic: Yes
Comments: Animals were immunized with a bovine TGF-beta2.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts with both TGF-beta1 and TGF-beta2, and can inhibit their biological activities.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
O.1 mM Non-Essential Amino Acids (NEAA)
fetal bovine serum to a final concentration of 10%
1D11.16.8細胞Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 22122: Dasch JR, et al. Monoclonal antibodies which bind both transforming growth factors .beta.1 and .beta.2 and methods of use. US Patent 5,571,714 dated Nov 5 1996
39037: Dasch JR, et al. 1D11.16.8細胞Monoclonal antibodies to transforming growth factor-beta and methods of use. US Patent 5,772,998 dated Jun 30 1998
39038: Dasch JR, et al. Monoclonal antibodies to transforming growth factor-beta and methods of use. US Patent 5,783,185 dated Jul 21 1998
