1C6 [LS77-1C6]細胞

生長狀態(tài)： 懸浮生長

ATCC Number： HB-12330?

是否是腫瘤細胞： 0

物種來源： 小鼠

數(shù)量： 大量

**類型： IgG1 kappa

運輸方式： 凍存運輸

Designations: 1C6 [LS77-1C6]

Depositors: LeukoSite, Inc.

1C6 [LS77-1C6]細胞Isotype: IgG1 kappa

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma)

Morphology:

Source: Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybrid

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. 1C6 [LS77-1C6]細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with a synthetic peptide corresponding to the first 37 N-terminal amino acids of CXCR3. Spleen cells were fused with Sp2/0 myeloma cells. The antibody recognizes surface expressed CXCR3, as judged by staining of CXCR3 transfected cells, but not untransfected cells. The antibody also stained human T cells and T cell clones that had been activated in vitro with PHA or anti-CD3. However, the anti-CXCR3 antibody did not react with neutrophils. The CXCR3 receptor is also known as the IP-10 receptor and the Mig receptor.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml.Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. 1C6 [LS77-1C6]細胞Do not allow the cell density to exceed 1 X 10(6) cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 71339: Loetscher M, et al. IP-10/Mig receptor designated CXCR3, antibodies, nucleic acids, and methods of use therefor. US Patent 6,184,358 dated Feb 6 2001

88724: Qin S, et al. The chemokine receptors CXCR3 and CCR5 mark subsets of T cells associated with certain inflammatory reactions. J. Clin. Invest. 101: 746-754, 1998. PubMed: 9466968

88725: Loetscher P, et al. 1C6 [LS77-1C6]細胞The ligands of CXC chemokine receptor 3, I-TAC, Mig, and IP10, are natural antagonists for CCR3. J. Biol. Chem. 276: 2986-2991, 2001. PubMed: 11110785

88726: Till S, et al. T cell phenotypes of the normal nasal mucosa: induction of Th2 cytokines and CCR3 expression by IL-4. J. Immunol. 166: 2303-2310, 2001. PubMed: 11160286

88727: Jones D, et al. Expression pattern of T-cell-associated chemokine receptors and their chemokines correlates with specific subtypes of T-cell non-Hodgkin lymphoma. Blood 96: 685-690, 2000. PubMed: 10887135