LL 29 (AnHa)細(xì)胞

數(shù)量： 大量

細(xì)胞形態(tài)： 成纖維樣

生長狀態(tài)： 貼壁生長

年限： 26 years

ATCC Number： CCL-134?

相關(guān)**： 其他**

細(xì)胞類型： 成纖維細(xì)胞

LL 29 (AnHa)細(xì)胞是否是腫瘤細(xì)胞： 0

物種來源： 人

運(yùn)輸方式： 凍存運(yùn)輸

器官來源： 肺

Designations: LL 29 (AnHa)

Depositors: RG Crystal

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: LL 29 (AnHa)細(xì)胞Homo sapiens

Morphology: fibroblast

Source: Organ: lung

Disease: idiopathic pulmonary fibrosis

Cell Type: fibroblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Age: 26 years

LL 29 (AnHa)細(xì)胞Gender: female

Ethnicity: Caucasian

Propagation: ATCC complete growth medium: Ham's F12K medium, 85%; fetal bovine serum, 15%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium and add fresh 0.25% trypsin, 0.03% EDTA for 1 to 2 minutes. Remove trypsin, EDTA and incubate the culture at 37C for 10 to 20 minutes with a small volume of trypsin - EDTA. LL 29 (AnHa)細(xì)胞Add fresh medium, aspirate and dispense into new flasks.

Preservation: Culture medium, 95%; DMSO, 5%