TFTA1 [3721.95.161.4]細(xì)胞
細(xì)胞形態(tài)： **樣
**類型： IgG1
運(yùn)輸方式： 凍存運(yùn)輸
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 小鼠
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
數(shù)量： 大量
ATCC Number： CRL-1771?
TFTA1 [3721.95.161.4]細(xì)胞Designations: TFTA1 [3721.95.161.4]
Depositors: R Fulton
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: TFTA1 [3721.95.161.4]細(xì)胞immunoglobulin; monoclonal antibody; against the A chain (A1 and A2) of the plant toxin ricin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with ricin A chain.
Spleen cells were fused with Sp2/5 myeloma cells (a subclone of Sp2/0-Ag14).
TFTA1 [3721.95.161.4]細(xì)胞The antibody does not cross-react with the ricin B chain.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1321: Fulton RJ, et al. TFTA1 [3721.95.161.4]細(xì)胞Purification of ricin A1, A2, and B chains and characterization of their toxicity. J. Biol. Chem. 261: 5314-5319, 1986. PubMed: 3957927
