Lo Wen細胞

運輸方式： 凍存運輸

生長狀態(tài)： 貼壁生長

是否是腫瘤細胞： 0

物種來源： 人

ATCC Number： CRL-1159?

相關(guān)**： 著色性干皮病

年限： 60 years

數(shù)量： 大量

器官來源： 皮膚

Lo Wen細胞細胞形態(tài)： 成纖維樣

Designations: Lo Wen

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: skin

Disease: Lo Wen細胞xeroderma pigmentosum, presumed heterozygote

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Age: 60 years

Gender: female

Comments: The tissue donor was a presumed heterozygote for xeroderma pigmentosum.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. Lo Wen細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Remove spent medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Lo Wen細胞Subculture every 6 to 8 days.