G2b.2細(xì)胞

ATCC Number： HB-109?

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

**類(lèi)型： IgG2b

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

運(yùn)輸方式： 凍存運(yùn)輸

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

G2b.2細(xì)胞Designations: G2b.2

Depositors: P Parham

Isotype: IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: G2b.2細(xì)胞hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against HLA DC1 (HLA DR)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with glycoprotein fraction from the Bristol 8 human B lymphoblastoid cell line.

Spleen cells were fused with NS-1 myeloma cells.

G2b.2細(xì)胞This line is a switch variant of Genox 3.53 (ATCC HB-103).

An IgG2a switch variant of Genox 3.53 also exists (G2a.5, ATCC HB-110).

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: RPMI 1640 medium with 0.1 mM hypoxanthine, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 994: Parham P, et al. G2b.2細(xì)胞Isolation of heavy chain class switch variants of a monoclonal anti-DC1 hybridoma cell line: effective conversion of noncytotoxic IgG1 antibodies to antibodies. Hum. Immunol. 8: 141-151, 1983. PubMed: 6417068