TH-1, Subline B1細胞

數(shù)量： 大量

細胞形態(tài)： 上皮樣

ATCC Number： CCL-50?

相關(guān)**： 正常

生長狀態(tài)： 貼壁生長

運輸方式： 凍存運輸

器官來源： 心臟

是否是腫瘤細胞： 0

TH-1, Subline B1細胞物種來源： 其他

Designations: TH-1, Subline B1

Depositors: HF Clark

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Terrapene carolina

Morphology: epithelial

Source: Organ: heart

Disease: TH-1, Subline B1細胞normal

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: This material is not available for international distribution due to the lack of historical data to support the issuance of a U.S. Fish and Wildlife Service Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES) export permit

Virus Resistance: poliovirus 1

Comments: Growth is inhibited at alkaline pH. Medium should be adjusted to neutrality after plating.

Propagation: TH-1, Subline B1細胞ATCC complete growth medium: Basal medium (Eagle) in Hanks' BSS, 90%; fetal bovine serum, 10%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: Every 10 to 14 days

Remove medium, add fresh 0.25% trypsin for 2 to 3 minutes, remove trypsin and allow the culture to stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.

References: 877: Clark HF, Karzon DT. Acquired tolerance to elevated temperatures in a poikilothermic cell line (terrapene heart, TH-1). Exp. Cell Res. 48: 269-275, 1967. PubMed: 6082311

878: Clark HF, Karzon DT. Terrapene heart (TH-1), a continuous cell line from the heart of the box turtle Terrapene carolina. Exp. Cell Res. 48: 263-268, 1967. PubMed: 4295486

57990: Huang CC, Clark HF. Chromosome changes in cell lines of the box turtle (Terrapene carolina) grown at two different temperatures. Can. J. Genet. Cytol. 9: 449-461, 1967. TH-1, Subline B1細胞PubMed: 6082587