G2a.5細(xì)胞

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

ATCC Number： HB-110?

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： **樣

**類型： IgG2a

Designations: G2a.5

G2a.5細(xì)胞Depositors: P Parham

Isotype: IgG2a

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against HLA DC1 (HLA DR)

Permits/Forms: G2a.5細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with glycoprotein fraction from the Bristol 8 human B lymphoblastoid cell line.

Spleen cells were fused with NS-1 myeloma cells.

This line is a switch variant of Genox 3.53 (ATCC HB-103).

An IgG2b switch variant of Genox 3.53 also exists (G2b.2, ATCC HB-109).

G2a.5細(xì)胞Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: RPMI 1640 medium with 0.1 mM hypoxanthine, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 994: Parham P, et al. Isolation of heavy chain class switch variants of a monoclonal anti-DC1 hybridoma cell line: effective conversion of noncytotoxic IgG1 antibodies to antibodies. Hum. Immunol. 8: 141-151, 1983. G2a.5細(xì)胞PubMed: 6417068