THB-7 [HB-7, HB7]細(xì)胞
數(shù)量： 大量
細(xì)胞形態(tài)： **樣
**類型： IgG1
生長狀態(tài)： 懸浮生長
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
ATCC Number： HB-136?
運(yùn)輸方式： 凍存運(yùn)輸
THB-7 [HB-7, HB7]細(xì)胞Designations: THB-7 [HB-7, HB7]
Depositors: TF Tedder
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: THB-7 [HB-7, HB7]細(xì)胞immunoglobulin; monoclonal antibody; against a human OKT-10 like molecule; against CD38
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with the BJAB human B cell line.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with a 45000 dalton OKT-10 like molecule on immature human lymphocytes, plasma cells and activated T cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: RPMI 1640 medium, 85%; fetal bovine serum, 15%
Temperature: 37.0°C
THB-7 [HB-7, HB7]細(xì)胞Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
References: 22717: Tedder TF, et al. THB-7 [HB-7, HB7]細(xì)胞Discontinuous expression of a membrane antigen (HB-7) during B lymphocyte differentiation. Tissue Antigens 24: 140-149, 1984. PubMed: 6440308
