12A5B7細(xì)胞

生長狀態(tài)： 懸浮生長

細(xì)胞形態(tài)： **樣

**類型： IgG

數(shù)量： 大量

ATCC Number： HB-8328?

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

12A5B7細(xì)胞Designations: 12A5B7

Depositors: Immunomedics, Inc.

Isotype: IgG

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: 12A5B7細(xì)胞immunoglobulin; monoclonal antibody; against human complement C1q

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Tumorigenic: Yes

Comments: Animals were immunized with purified human C1q.

12A5B7細(xì)胞Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 3547: Reckel RP, et al. 12A5B7細(xì)胞Method for detecting immune complexes in serum. US Patent 4,595,654 dated Jun 17 1986