TIMI.4細(xì)胞
ATCC Number： TIB-37?
相關(guān)**： **瘤
細(xì)胞形態(tài)： **樣
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 小鼠
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
品系： C57BL/6
數(shù)量： 大量
TIMI.4細(xì)胞運(yùn)輸方式： 凍存運(yùn)輸
器官來(lái)源： 胸腺
Designations: TIMI.4
Depositors: R Hyman
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Strain: C57BL/6
TIMI.4細(xì)胞Organ: thymus
Disease: lymphoma
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Antigen Expression: H-2b; Thy-1.2
Comments: Derived from a radiation leukemia virus induced thymoma.
The line is sensitive to cortisol (0.001 mM) and PHA (0.02 mg/ml).
TL antigen is not expressed.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. TIMI.4細(xì)胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1104: Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304
1129: Hyman R. Studies on surface antigen variants. Isolation of two complementary variants for Thy 1.2. J. Natl. Cancer Inst. 50: 415-422, 1973. PubMed: 4702114
22528: Ralph P, Nakoinz I. TIMI.4細(xì)胞Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714
