Gam Per細胞

數(shù)量： 大量

器官來源： 皮膚

年限： 63 years

ATCC Number： CRL-1326?

相關(guān)**： Ehlers-Danlos綜合征子

生長狀態(tài)： 貼壁生長

運輸方式： 凍存運輸

是否是腫瘤細胞： 0

物種來源： 人

細胞形態(tài)： 成纖維樣

Gam Per細胞Designations: Gam Per

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: skin

Disease: Ehlers-Danlos syndrome, possible heterozygote

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Gam Per細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Age: 63 years

Gender: female

Comments: The tissue donor was a possible heterozygote for Ehlers-Danlos syndrome, and was the grandmother of Jo Per (ATCC CRL-1332), No Per (ATCC CRL-1327) and Cor Per II (ATCC CRL-1243).

Gam Per細胞Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: 2 to 3 times per week

Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Gam Per細胞Subculture every 8 to 10 days.