LP3IIG2細(xì)胞

ATCC Number： HB-8472?

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

數(shù)量： 大量

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： **樣

**類型： IgG2a

LP3IIG2細(xì)胞Designations: LP3IIG2

Depositors: Genetic Systems Corp.

Isotype: IgG2a

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: LP3IIG2細(xì)胞immunoglobulin; monoclonal antibody; against Legionella pneumophila

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Comments: Animals were immunized with Legionella pneumophila.

Spleen cells were fused with NS-1 myeloma cells.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.

LP3IIG2細(xì)胞Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 3893: Goldstein LC, Gosting LH. LP3IIG2細(xì)胞Method and composition for the detection and diagnosis of Legionella pneumophila. US Patent 4,851,333 dated Jul 25 1989