LS 102.9細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

ATCC Number： HB-97?

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

Designations: LS 102.9

Depositors: P Marrack, J Kappler

Biosafety Level: 1

LS 102.9細(xì)胞Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (lymphoma) deposited as mouse (B cell); mouse (lymphoma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. LS 102.9細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: The LS 102.9 bears surface I-A d,s and I-E d molecules and can present antigen to appropriate I region restricted T cell hybridomas.

The line was produced by fusing A20.2J lymphoma cells (I-Ad, I-Ed) with T cell depleted spleen cells from B10.S(7R) mice (I-As, I-Es).

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 10%

LS 102.9細(xì)胞1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere

0.05 mM 2-Mercaptoethanol

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 3 to 4 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml Maintain pH below 7.2.

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X

References: 958: Kappler J, et al. LS 102.9細(xì)胞Antigen presentation by Ia+ B cell hybridomas to H-2 restricted T cell hybridomas. Proc. Natl. Acad. Sci. USA 79: 3604-3607, 1982. PubMed: 6980413