TIMI.4G.1.3細胞

細胞類型： T**細胞

數(shù)量： 大量

是否是腫瘤細胞： 0

物種來源： 小鼠

運輸方式： 凍存運輸

細胞形態(tài)： **樣

品系： C57BL/6

ATCC Number： TIB-38?

相關(guān)**： **瘤

TIMI.4G.1.3細胞生長狀態(tài)： 懸浮生長

Designations: TIMI.4G.1.3

Depositors: R Hyman

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast

Source: Disease: lymphoma

Strain: C57BL/6

Cell Type: TIMI.4G.1.3細胞T lymphocyte;

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Antigen Expression: H-2b; Thy-1.2

Comments: This line was derived from TIMI.4 (ATCC TIB-37).

The cells are resistant to 0.1 mM 6-thioguanine.

Tested and found negative for ectromelia virus (mousepox).

Propagation: TIMI.4G.1.3細胞ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1129: Hyman R. Studies on surface antigen variants. TIMI.4G.1.3細胞Isolation of two complementary variants for Thy 1.2. J. Natl. Cancer Inst. 50: 415-422, 1973. PubMed: 4702114