2A2-6E10-1D8 [1D8]細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： **樣
**類型： IgG1
ATCC Number： HB-12465?
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
生長狀態(tài)： 懸浮生長
數(shù)量： 大量
2A2-6E10-1D8 [1D8]細(xì)胞Designations: 2A2-6E10-1D8 [1D8]
Depositors: University of Massachusetts Medical Center
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: 2A2-6E10-1D8 [1D8]細(xì)胞immunoglobulin; monoclonal antibody; against Norwalk Virus
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Tumorigenic: Yes
Comments: Animals were immunized with stool-derived Norwalk virus.
Spleen cells were fused with Sp2/0 mouse myeloma cells.
The antibody is directed against an epitope of the 58 kDa antigen of Norwalk Virus. [56174]
The antibody specifically binds to Norwalk virus particles found in human stool specimens from naturally occurring Norwalk virus outbreaks. [56174]
The antibody reacts with the prototype 8FIIa strain of Norwalk virus in an enzyme immunoassay (EIA) for detecting Norwalk virus in stool specimens. 2A2-6E10-1D8 [1D8]細(xì)胞[56263]
No cross-reactions were seen in stools containing other caliciviruses or in stools containing rotaviruses, astroviruses, or enteric adenoviruses. [56263]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Attached cells may need to be shaken loose.
Preservation: culture medium 95%; DMSO, 5%
Related Products: 2A2-6E10-1D8 [1D8]細(xì)胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
References: 56174: Herrmann JE, Blacklow NR. Monoclonal antibodies for detecting Norwalk virus. US Patent 5,861,241 dated Jan 19 1999
56263: Herrmann JE, et al. Monoclonal antibodies for detection of Norwalk virus antigen in stools. J. Clin. Microbiol. 33: 2511-2513, 1995. PubMed: 7494063
56265: Brinker JP, et al. Detection of Norwalk virus and other genogroup 1 human caliciviruses by a monoclonal antibody, recombinant-antigen-based immunoglobulin M capture enzyme immunoassay. J. Clin. Microbiol. 36: 1064-1069, 1998. PubMed: 9542938
