tk- ts13細胞

運輸方式： 凍存運輸

是否是腫瘤細胞： 0

物種來源： 其他

ATCC Number： CRL-1632?

生長狀態(tài)： 貼壁生長

器官來源： 腎臟

數(shù)量： 大量

Designations: tk- ts13

tk- ts13細胞Depositors: R Baserga

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mesocricetus auratus

Morphology:

Source: Organ: kidney

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Applications: transfection host

Can be transfected at a high rate of efficiency.

Comments: Thymidine kinase-deficient derivative of ts13, originally isolated as a temperature-sensitive mutant of the baby hamster kidney cell line BHK-21.

The tk-ts13 cell line is a derivative of BHK-21.

The cells are temperature sensitive for growth, and are thymidine kinase deficient (TK-).

The line is an efficient host for transfection studies.

The cells should be grown at 34C.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

tk- ts13細胞Temperature: 34.0°C

Subculturing: Remove medium, rinse with fresh 0.25% trypsin, 0.02% versene solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes).

Add fresh medium, aspirate and dispense into new flasks Inoculate new cultures at 2 X 10 exp4 cells/sq cm.

References: 26118: Talavera A, Basilico C. Temperature sensitive mutants of BHK cells affected in cell cycle progression. J. Cell. Physiol. 92: 425-436, 1977. PubMed: 903382

32471: Black ME, et al. Creation of drug-specific herpes simplex virus type 1 thymidine kinase mutants for gene therapy. Proc. Natl. Acad. Sci. USA 93: 3525-3529, 1996. PubMed: 8622970

41386: Shen YM, et al. tk- ts13細胞Superimposition of temperature regulation on yeast promoters. Mol. Cell. Biol. 2: 1145-1154, 1982. PubMed: 6294505