TK6細(xì)胞

細(xì)胞類(lèi)型： **母細(xì)胞

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 人

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

細(xì)胞形態(tài)： **樣

ATCC Number： CRL-8015?

相關(guān)**： 其他**

運(yùn)輸方式： 凍存運(yùn)輸

器官來(lái)源： 脾

年限： 5 years

TK6細(xì)胞Designations: TK6

Depositors: Massachusetts Institute of Technology

Biosafety Level: 2

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Organ: spleen

Disease: hereditary spherocytosis

Cell Type: lymphoblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. TK6細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 11

D16S539: 11,12

D5S818: 12,13

D7S820: 9,11

THO1: 8,9.3

TPOX: 8,11

vWA: 17,20

Age: 5 years

Gender: male

Comments: This line is derivative of the WIL-2 cell line.

TK6細(xì)胞The cells are heterozygous at the thymidine kinase (TK) locus, and can be

used to quantitatively detect forward mutation at 3 loci (resistance to trifluorothymidine (tk locus).

Resistance to thioguanine (hprt locus), and resistance to ouabain (Na/K ATPase].

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Maintain the cell concentration between 2 X 10(5) and 1 X 10(6) cells/ml.

Medium Renewal: Every 2 to 3 days (or as necessary to maintain the cell concentration between 2 X 10(5) and 1 X 10(6) cells/ml)

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

WIL2-NS, ATCC CRL-8155

WIL2-S, ATCC CRL-8885

recommended serum:ATCC 30-2020

LTR228 , ATCC HB-8502

References: 4000: TK6細(xì)胞Skopek TR, et al. Assay for mutagenesis in heterozygous diploid human lymphoblasts. US Patent 4,302,535 dated Nov 24 1981

22402: Skopek TR, et al. Isolation of a human lymphoblastoid line heterozygous at the thymidine kinase locus: possibility for a rapid human cell mutation assay. Biochem. Biophys. Res. Commun. 84: 411-416, 1978. PubMed: 214074

22789: Levy JA, et al. Human lymphoblastoid lines from lymph node and spleen. Cancer 22: 517-524, 1968. PubMed: 5673231

32932: Dolganov GM, et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair. Mol. Cell. Biol. 16: 4832-4841, 1996. PubMed: 8756642