2B5.3細(xì)胞

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

生長狀態(tài)： 懸浮生長

運輸方式： 凍存運輸

細(xì)胞形態(tài)： **樣

**類型： IgG2b; kappa light chain

ATCC Number： CRL-1960?

Designations: 2B5.3

Depositors: MF Heyworth

Isotype: IgG2b; kappa light chain

2B5.3細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against Giardia muris trophozoites

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Comments: Animals were immunized with G. muris trophozoites.

Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells.

2B5.3細(xì)胞The antibody reacts with G. muris trophozoite surfaces.

Unlike 3C7.2 (ATCC CRL-1959), 1A3.1 (ATCC CRL-1961) and 1B1.1, the 2B5.3 antibody specifically recognizes trophozoite antigens of apparent molecular weight 31000 and 35000 on western blots.

The antibody immunoprecipitates two polypeptides with apparent molecular weights of 30000.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%;NCTC 109 medium, 10%;(Add 1 mM oxaloacetic acid, 0.45 mM sodium pyruvate, 4 mM L-glutamine, 0.1 mM hypoxanthine, 0.01 mM thymidine and 10 units/ml bovine insulin to this mixture);horse serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 22728: Heyworth MF, et al. 2B5.3細(xì)胞Generation and characterization of monoclonal antibodies against Giardia muris trophozoites. Immunology 68: 341-345, 1989. PubMed: 2592009