Genox 3.53細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

ATCC Number： HB-103?

細(xì)胞形態(tài)： **樣

**類型： IgG1

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

數(shù)量： 大量

Genox 3.53細(xì)胞Designations: Genox 3.53

Depositors: P Parham

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products:Genox 3.53細(xì)胞 immunoglobulin; monoclonal antibody; against DQw1 (DC1, HLA DR)

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Comments: Animals were immunized with glycoproteins from the Bristol 8 human B lymphoblastoid cell line.

Spleen cells were fused with NS-1 myeloma cells.

The antibody reacts with the DQw1 (DC10) determinant of the HLA D region.

Genox 3.53細(xì)胞Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 951: Brodsky FM, et al. Genox 3.53細(xì)胞Monoclonal antibodies to HLA -- DRw determinants. Tissue Antigens 16: 30-48, 1980. PubMed: 6162228