28-6-20細(xì)胞

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

**類型： rat IgG1 and 2a

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CRL-2489?

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 大鼠

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

Designations: 28-6-20

28-6-20細(xì)胞Depositors: DG Sieckmann

Isotype: rat IgG1 and 2a

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybrid

Cellular Products: immunoglobulin; monoclonal antibody; against MOPC167 idiotype (V kappa 24)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. 28-6-20細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: This cell line was deposited at the ATCC by Donna G. Sieckmann, Naval Medical Research Center. The line is distributed for academic research purposes only. The Naval Medical Research Center releases the line subject to the following: 1.) The line or its products must not be distributed to third parties. Commercial interests are the exclusive property of the Naval Medical Research Center. 2.) Any proposed commercial use of the cell line including any use by a for-profit entity must first be negotiated with the Commanding Officer, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910-7500.

Isolation: Isolation date: 1990

Comments: The 28-2-7 (ATCC CRL-2487) and 28-6-20 (ATCC CRL-2489) antibodies bind the V kappa 24 light chain idiotypic specificity of MOPC167.

Animals were immunized with MOPC167 and HPCM27 anti-phosphocholine (PC) antibodies. MOPC167 is an IgA myeloma specific for PC and HPCM27 is an IgM myeloma specific for PC.

Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.

The antibodies are specific for PC-binding proteins of VH1/V kappa 24 heavy and light chains of various isotypes. They do not bind VH1/V kappa 22, VH1/V kappa 8, or VH1/V kappa 1 PC-binding proteins or other IgA or IgM myeloma proteins. [40008]

These antibodies can be used as typing reagents for mouse IgA bearing the V kappa 24 idiotypic specificity.

They can be used in typing the 207-4 immunoglobulin transgenic mice that carry the VH1/V kappa 24 gene segment of the IgA PC specific MOPC167 myeloma.

Propagation: 28-6-20細(xì)胞ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.

Interval: Maintain cultures at a cell concentration between 5 X 10(4) and 1 X 10(6) cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: 28-6-20細(xì)胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020

References: 40008: Sieckmann DG, et al. Anti-idiotype monoclonal antibodies specific for the MOPC167 anti-phosphocholine transgene-encoded antibody. Hybridoma 16: 503-511, 1997. PubMed: 9455702