NRbM細胞

運輸方式： 凍存運輸

數量： 大量

是否是腫瘤細胞： 0

物種來源： 小鼠

細胞形態(tài)： **樣

**類型： IgG1

生長狀態(tài)： 懸浮生長

ATCC Number： CRL-1839?

Designations: NRbM

Depositors: R Wistar, C Cole

Isotype: IgG1

NRbM細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against rabbit IgM

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. NRbM細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with rabbit IgM.

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

The antibody reacts with rabbit IgM but not with other rabbit immunoglobulin classes.

Propagation: ATCC complete growth medium: NRbM細胞Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

NRbM細胞References: 58339: Cole C, Wistar R Jr.. Monoclonal antibody anti-rabbit IgM, NR6M. Monoclonal Antibody News 7: 15-16, 1989.