運輸方式： 凍存運輸
生長狀態(tài)： 懸浮生長
數(shù)量： 大量
ATCC Number： HB-275?
細胞形態(tài)： **樣
**類型： IgG1
是否是腫瘤細胞： 0
物種來源： 小鼠
Designations: CC58
Depositors: CJ Howard
Isotype: IgG1
Biosafety Level: 1
CC58細胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against bovine CD8 (BoCD8)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with bovine lymphocytes and thymocytes.
Spleen cells were fused with NS1 myeloma cells.
CC58細胞The antibody is specific for bovine CD8 T lymphocyte antigen.
It appears to be reactive only with the alpha/beta heterodimer and not the alpha/alpha homodimer.
This antibody was included in the panel of the International Workshop on Bovine, Sheep and Goat Leukocyte Differentiation Antigens in 1989.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: CC58細胞Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(5) cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
References: 22775: Hein WR, et al. Epitopes of the T19 lymphocyte surface antigen are extensively conserved in ruminants. Vet. Immunol. Immunopathol. 27: 173-181, 1991. CC58細胞PubMed: 1708546
