K-12 MG1655大腸桿菌克隆菌使用說(shuō)明：
滬震質(zhì)粒平臺(tái)的各批次質(zhì)粒菌株發(fā)貨前均經(jīng)過(guò)嚴(yán)格的多重驗(yàn)證，如存在質(zhì)量問(wèn)題，請(qǐng)?jiān)谑盏疆a(chǎn)品三個(gè)月內(nèi)通知我司。收到甘油菌后，請(qǐng)及時(shí)在固體平板上劃線培養(yǎng)，挑取單菌落接種到液體培養(yǎng)基中，震蕩培養(yǎng)后使用并保存甘油菌。如需獲取其他詳細(xì)信息請(qǐng)登錄我司官網(wǎng)聯(lián)系QQ客服查詢。
基因型：
F- lambda- ilvG- rfb-50 rph-1(NC_000913)
K-12 MG1655大腸桿菌克隆菌基本信息：
抗性：無(wú)
培養(yǎng)基：LB
菌株類別：大腸桿菌
培養(yǎng)條件：37℃，有氧，LB
質(zhì)粒轉(zhuǎn)化：42℃熱激
保存方式：20%甘油，-20℃
基本應(yīng)用：用于基因克隆
備注：
K-12 MG1655大腸桿菌克隆菌菌株簡(jiǎn)介：
K-12 MG1655是一株K系列的大腸桿菌，經(jīng)檢測(cè)無(wú)Amp和Kan抗性，可用LB培養(yǎng)基，37℃培養(yǎng)，其代謝類型是異養(yǎng)兼性厭氧型，可用于代謝研究、基因編輯等實(shí)驗(yàn)。
The original K-12 wild-type strain from the Stanford collection contained both the F plasmid and phage lambda. MG1655 was constructed by curing that strain using acridine orange and UV.This strain was sequenced by the Blattner laboratory because it approximates wild-type E. coli and "has been maintained as a laboratory strain with minimal genetic manipulation, having only been cured of the temperate bacteriophage lambda and F plasmid by means of ultraviolet light and acridine orange, respectively." (Blattner, et al. 1997). The mutations listed in the genotype are present in most K-12 strains and were probably acquired early in the history of the laboratory strain. A frameshift at the end of rph results in decreased pyrE expression and a mild pyrimidine starvation, such that the strain grows 10 to 15% more slowly in pyrimidine-free medium than in medium containing uracil (Jensen 1993). The ilvG- mutation is a frameshift that knocks out acetohydroxy acid synthase II (Lawther, et al. 1982). The rfb-50 mutation is an IS5 insertion that results in the absence of O-antigen synthesis (Liu and Reeves 1994).
MG1655 was derived and named by Mark Guyer from strain W1485, which was derived in Joshua Lederberg's lab from a stab-culture descendant of the original K-12 isolate. This original E. coli strain K-12 was obtained from a stool sample of a diphtheria patient in Palo Alto, CA in 1922 (Bachmann, B., pp. 2460-2488 in Neidhardt et al.1996, Escherichia coli and Salmonella: Cellular and Molecular Biology, ASM Press).MG1655 grows on LB and M9 minimal medium (+ Glucose + 1ug/ml thiamine).
K-12 MG1655大腸桿菌克隆菌注意事項(xiàng)：
1、為了您的**和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。本產(chǎn)品僅可用于實(shí)驗(yàn)室研究，不能用于動(dòng)物，人體以及作為食品添加劑等用途。
2、使用甘油菌時(shí)可不完全融解，在甘油菌表面蘸取少量涂布固體瓊脂平板即可，也可完全融解后使用，但隨著凍融次數(shù)的增加，菌株的活力會(huì)逐漸下降。hz1094 木糖賴氨酸去氧膽酸鹽瓊脂（XLD） 250g 選擇性培養(yǎng)基，用于分離志賀氏菌屬，也可用于分離沙門氏菌屬
yblose Lysine Desoybcholate Medium
hz1095 志賀氏菌增菌肉湯 250g 用于志賀氏菌增菌培養(yǎng)
Shigella Broth
hz1096 WS瓊脂 250g 用于沙門氏菌的選擇性分離（GB標(biāo)準(zhǔn)）
WS Salmonella Agar
hz1097 氯化鎂孔雀綠增菌液 250g 用于沙門氏菌選擇性增菌培養(yǎng)(ISO標(biāo)準(zhǔn))
Rappaport Vassiliadis Medium
hz1098 酚紅煌綠瓊脂 250g 用于沙門氏菌分離培養(yǎng)（GB標(biāo)準(zhǔn)）
Phenolphthalein Brilliant Green Agar
hz1099 葡萄糖銨培養(yǎng)基 250g 用于志賀氏菌的葡萄糖銨試驗(yàn)（GB標(biāo)準(zhǔn)）
Ammonium Dextrose Medium
hz1100 葡萄糖半固體培養(yǎng)基 250g 用于志賀氏菌的復(fù)合生化試驗(yàn)（GB標(biāo)準(zhǔn)）
Dextrose Semisolid Medium
hz1101 動(dòng)力-吲哚-尿素培養(yǎng)基基礎(chǔ)（MIU） 250g 用于**的復(fù)合生化試驗(yàn)
Motility Indol Urea Medium Base
hz1102 中國(guó)藍(lán)瓊脂培養(yǎng)基 250g 用于腸道菌選擇性分離培養(yǎng)
China Blue Medium

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