SW 900細胞

運輸方式： 凍存運輸

生長狀態(tài)： 貼壁生長

年限： grade IV

器官來源： 肺

細胞形態(tài)： 上皮樣

ATCC Number： HTB-59?

相關(guān)**： 鱗狀細胞癌

是否是腫瘤細胞： 1

物種來源： 人

數(shù)量： 大量

Designations: SW 900 [SW-900, SW900]

Depositors: A Leibovitz

SW 900細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: lung

Tumor Stage: grade IV

Disease: squamous cell carcinoma

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Tumorigenic: Yes

Antigen Expression: Blood type A; Rh+

DNA Profile (STR): Amelogenin: X

CSF1PO: 11

D13S317: 8

SW 900細胞D16S539: 11

D5S818: 11

D7S820: 11,12

THO1: 8

TPOX: 11

vWA: 16

Cytogenetic Analysis: hypotriploid; modal number = 56. The rate of higher ploidies was 21.8%. Cells in this line have complex karyotypes., Over 30 marker chromosomes were found in 5 metaphase karyotypes. The marker chromosomes, t(1q;10q), t(1q;22q), t(2q;3q), der (3)t(2;3)(p11;q29), der (7)t(7;17) (q36;q21) and about 20 others were common to most cells., Among these der (7) was generally paired. Normal N3, N5, N12, N13, N14 and N17 were absent. The X was single and the Y was not found on fluorescence observation.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 2

PGM1, 1

PGM3, 2

Age: 53 years

Gender: male

Ethnicity: Caucasian

Comments: SW 900細胞The SW 900 cell line was initiated by A. Leibovitz in January 1975 from biopsy tissue of a squamous cell carcinoma.

The tumor originated in the lung of a 53 year old male Caucasian.

The histopathology of the surgical specimen was determined to be grade IV carcinoma.

A frozen ampule at passage 15 was received at the ATCC in January, 1982.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 100%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: recommended serum:ATCC 30-2020

References: 22536: SW 900細胞Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

22551: Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474