NCI-H498細(xì)胞

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)，少量貼壁

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

器官來(lái)源： 盲腸

年限： 56 years

細(xì)胞形態(tài)： 上皮樣

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

ATCC Number： CCL-254?

相關(guān)**： 大腸癌

Designations: NCI-H498 [H498]

NCI-H498細(xì)胞Depositors: AF Gazdar

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, with some loosely adherent cells

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: cecum

Disease: colorectal adenocarcinoma

Cellular Products: carcinoembryonic antigen (CEA), 826 ng/ml per 10 exp6 cells per 10 days; mucin

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Tumorigenic: Yes

Antigen Expression: Rh+; CA19-9 antigen; TAG-72 antigen

DNA Profile (STR): NCI-H498細(xì)胞Amelogenin: X

CSF1PO: 12

D13S317: 9,11

D16S539: 9,11

D5S818: 13

D7S820: 8,11

THO1: 7

TPOX: 8

vWA: 15

Cytogenetic Analysis: modal number = 51; range = 47 to 54.

This is a hyperdiploid human cell line. The modal chromosome number is 51, occurring in 42% of the cells. The rate of higher ploidies is 5%. The 2q+ is the only marker chromosome detected and common to all cells. There are four copies for N7; 3 copies each for N8, N13 and N22, and a single copy for the X.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 1

PGM1, 1

PGM3, 1-2

NCI-H498細(xì)胞Age: 56 years

Gender: male

Ethnicity: Caucasian

Comments: This line was derived from a metastasis in the peritoneum obtained from a patient prior to therapy.

The cells form glandular structures and secrete mucin.

The cells are positive for Dopa decarboxylase, CA19-9 and TAG-72 antigens and CEA.

Although the patient from which this cell was derived was blood type A, the cell line does not react with anti type B antibody and has very low to undetectable levels of alpha-N-acetylgalactosaminyltransferase activity.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Alternatively, the cells may be collected by centrifugation and then resuspended at 2 to 4 X 10(4) viable cells/ml. When the cell concentration becomes heavy with large clumps, aspirate well and and dilute approximately 1:10. If the clumps are allowed to get too large, most of the cells will die.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: NCI-H498細(xì)胞Complete growth medium supplemented with 10% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 23066: Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249

23074: Dahiya R, et al. ABH blood group antigen expression, synthesis, and degradation in human colonic adenocarcinoma cell lines. Cancer Res. 49: 4550-4556, 1989. PubMed: 2545345