97人妻人人做人碰人人爽,亚洲AV无码一区二区乱孑伦AS,免费人成视频在线观看 ,99久久精品日本一区二区免费

產(chǎn)品資料

NCI-H498細(xì)胞

如果您對(duì)該產(chǎn)品感興趣的話,可以
產(chǎn)品名稱: NCI-H498細(xì)胞
產(chǎn)品型號(hào): NCI-H498
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無(wú)相關(guān)文檔

簡(jiǎn)單介紹

NCI-H498細(xì)胞應(yīng)如何避免細(xì)胞污染,細(xì)胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因?yàn)闊o(wú)菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細(xì)胞等。嚴(yán)格之無(wú)菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細(xì)胞來(lái)源和培養(yǎng)基配制是減低污染之*好方法。NCI-H498細(xì)胞何時(shí)須更換培養(yǎng)基?視細(xì)胞生長(zhǎng)密度而定,或遵照細(xì)胞株基本數(shù)據(jù)上之更換時(shí)間,按時(shí)更換培養(yǎng)基即可。


NCI-H498細(xì)胞  的詳細(xì)介紹

NCI-H498細(xì)胞

生長(zhǎng)狀態(tài): 懸浮生長(zhǎng),少量貼壁

運(yùn)輸方式: 凍存運(yùn)輸

數(shù)量: 大量

器官來(lái)源: 盲腸

年限: 56 years

細(xì)胞形態(tài): 上皮樣

是否是腫瘤細(xì)胞: 1

物種來(lái)源: 人

ATCC Number: CCL-254?

相關(guān)**: 大腸癌

Designations: NCI-H498 [H498]

NCI-H498細(xì)胞Depositors: AF Gazdar

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, with some loosely adherent cells

Organism: Homo sapiens

Morphology: epithelial


Source: Organ: cecum

Disease: colorectal adenocarcinoma

Cellular Products: carcinoembryonic antigen (CEA), 826 ng/ml per 10 exp6 cells per 10 days; mucin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Antigen Expression: Rh+; CA19-9 antigen; TAG-72 antigen

DNA Profile (STR): NCI-H498細(xì)胞Amelogenin: X

CSF1PO: 12

D13S317: 9,11

D16S539: 9,11

D5S818: 13

D7S820: 8,11

THO1: 7

TPOX: 8

vWA: 15

Cytogenetic Analysis: modal number = 51; range = 47 to 54.

This is a hyperdiploid human cell line. The modal chromosome number is 51, occurring in 42% of the cells. The rate of higher ploidies is 5%. The 2q+ is the only marker chromosome detected and common to all cells. There are four copies for N7; 3 copies each for N8, N13 and N22, and a single copy for the X.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 1

PGM1, 1

PGM3, 1-2

NCI-H498細(xì)胞Age: 56 years

Gender: male

Ethnicity: Caucasian

Comments: This line was derived from a metastasis in the peritoneum obtained from a patient prior to therapy.

The cells form glandular structures and secrete mucin.

The cells are positive for Dopa decarboxylase, CA19-9 and TAG-72 antigens and CEA.

Although the patient from which this cell was derived was blood type A, the cell line does not react with anti type B antibody and has very low to undetectable levels of alpha-N-acetylgalactosaminyltransferase activity.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Alternatively, the cells may be collected by centrifugation and then resuspended at 2 to 4 X 10(4) viable cells/ml. When the cell concentration becomes heavy with large clumps, aspirate well and and dilute approximately 1:10. If the clumps are allowed to get too large, most of the cells will die.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: NCI-H498細(xì)胞Complete growth medium supplemented with 10% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 23066: Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249

23074: Dahiya R, et al. ABH blood group antigen expression, synthesis, and degradation in human colonic adenocarcinoma cell lines. Cancer Res. 49: 4550-4556, 1989. PubMed: 2545345

滬公網(wǎng)安備 31011702004356號(hào)

丰满少妇被猛烈进入毛片| 欧美大肥婆大肥bbbbb| 国产成人无码AV在线播放不卡 | 免费看又黄又无码的网站| 久久国产成人精品国产成人亚洲| 51妺嘿嘿午夜福利| 高潮太激动抽搐两眼翻白| 亚洲小说区图片区另类春色| 肉乳床欢无码a片120秒厨房里| 亚洲色精品AⅤ一区区三区| 国产高清无密码一区二区三区 | 天堂…在线最新版资源| 国产真实乱人偷精品视频| 国产后入清纯学生妹| 久久精品亚洲AV无码四区妖精| 一受多攻同做h嗯啊巨肉| 亚洲AV无码乱码在线观看富二代| 高清无码在线观看| 少妇高潮毛片色欲AVA片| JAPANESE熟女俱乐部| 夜夜夜夜猛噜噜噜噜噜试看| 性一交—乱一性一A片在线播放| 联系附近成熟妇女| 豪妇荡乳1一5全集| 波多野结AV衣东京热无码专区| 人人妻人人添人人爽欧美一区| 嫩bbb槡bbbb槡bbbb| 黎明破晓前电视剧全集免费观看 | 久久成人国产精品免费| 久久精品一区二区三区中文字幕| 国产熟妇高潮叫床视频播放| 免费午夜爽爽爽WWW视频十八禁| 极品少妇高潮啪啪av无码吴梦梦| 欧产日产国色天香区别视频| 欧洲精品久久久AV无码电影| 亚洲一区二区三区日本久久九| 久久日产一线二线三线| 富婆被躁30分钟黑人大战| 国产人成视频在线观看| 欧美性生交XXXXX久久久| 在线观看国产一区二区三区|