Hs 696細(xì)胞

年限： 43 years

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

ATCC Number： HTB-151?

相關(guān)**： 腺癌

細(xì)胞形態(tài)： 上皮樣

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

Designations: Hs 696

Depositors: RB Owens

Hs 696細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Disease: adenocarcinoma

Derived from metastatic site: bone (sacrum)

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Tumorigenic: No

Cytogenetic Analysis: Y chromosome present

Isoenzymes: AK-1, 1

ES-D, 1

Hs 696細(xì)胞G6PD, B

GLO-I, 1-2

Me-2, 1

PGM1, 1

PGM3, 1

Age: 43 years

Gender: male

Ethnicity: Asian

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: Hs 696細(xì)胞A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22534: Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412

22543: Smith HS. In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. Hs 696細(xì)胞PubMed: 283258