Hs 895.T細(xì)胞

細(xì)胞形態(tài)： 成纖維樣

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

運(yùn)輸方式： 凍存運(yùn)輸

年限： 48 years

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

數(shù)量： 大量

器官來(lái)源： 皮膚

ATCC Number： CRL-7637?

相關(guān)**： 黑色素瘤

Hs 895.T細(xì)胞Designations: Hs 895.T

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: skin

Disease: melanoma

Derived from metastatic site: lung

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Hs 895.T細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: Thus, the two lines provide tumor and normal counterparts for comparative in vitro studies.

DNA Profile (STR): Amelogenin: X

CSF1PO: 11

D13S317: 12

D16S539: 12,14

D5S818: 11,12

D7S820: 10,12

THO1: 6,7

TPOX: 8,11

vWA: 18

Age: 48 years

Gender: female

Ethnicity: Caucasian

Comments: Hs 895.T細(xì)胞Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.

This melanoma cell line was isolated from the same patient as Hs 895.Sk (ATCC CRL-7636), a normal skin fibroblast. Thus, the two lines provide tumor and normal counterparts for comparative in vitro studies.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Hs 895.T細(xì)胞recommended serum:ATCC 30-2002

Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2020

derived from same individual: ATCC CRL-7636, Hs 895