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Hs 936.T(C1)細(xì)胞

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產(chǎn)品名稱: Hs 936.T(C1)細(xì)胞

產(chǎn)品型號(hào): Hs 936.T(C1)

產(chǎn)品展商: HZbscience

產(chǎn)品文檔: 無相關(guān)文檔

簡(jiǎn)單介紹

Hs 936.T(C1)細(xì)胞應(yīng)如何避免細(xì)胞污染，細(xì)胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因?yàn)闊o菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細(xì)胞等。嚴(yán)格之無菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細(xì)胞來源和培養(yǎng)基配制是減低污染之*好方法。Hs 936.T(C1)細(xì)胞何時(shí)須更換培養(yǎng)基？視細(xì)胞生長(zhǎng)密度而定，或遵照細(xì)胞株基本數(shù)據(jù)上之更換時(shí)間，按時(shí)更換培養(yǎng)基即可。

Hs 936.T(C1)細(xì)胞 的詳細(xì)介紹

Hs 936.T(C1)細(xì)胞

ATCC Number： CRL-7687?

相關(guān)**：黑色素瘤

運(yùn)輸方式：凍存運(yùn)輸

數(shù)量：大量

器官來源：皮膚

細(xì)胞形態(tài)：上皮樣

生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)

是否是腫瘤細(xì)胞： 1

Hs 936.T(C1)細(xì)胞物種來源：人

Designations: Hs 936.T(C1)

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: skin

Disease: melanoma

Hs 936.T(C1)細(xì)胞Cellular Products: melanin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Gender: male

Comments: Clone of ATCC CRL-7686.

Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.

Please see the NBL Repository description.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. Hs 936.T(C1)細(xì)胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: 1:2 to 1:4

Medium Renewal: every 2 to 3 days

Preservation: Freeze medium: Complete growth medium, 95%; DMSO 5%

Storage temperature:Hs 936.T(C1)細(xì)胞 liquid nitrogen vapor

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

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