C-4 II細(xì)胞
數(shù)量： 大量
ATCC Number： CRL-1595?
相關(guān)**： 腫瘤
器官來源： 宮頸
細(xì)胞形態(tài)： 上皮樣
是否是腫瘤細(xì)胞： 1
物種來源： 人
年限： 41 years
生長狀態(tài)： 貼壁生長
運(yùn)輸方式： 凍存運(yùn)輸
Designations: C-4 II
Depositors: N Auersperg
C-4 II細(xì)胞Biosafety Level: 2 [Cells contain human papilloma virus ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: cervix
Disease: carcinoma
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DNA Profile (STR): Amelogenin: X
CSF1PO: 12
D13S317: 11,12
D16S539: 11
D5S818: 9,11
D7S820: 10,11
THO1: 9,9.3
C-4 II細(xì)胞TPOX: 10,11
vWA: 14
Isoenzymes: G6PD, B
Age: 41 years
Gender: female
Ethnicity: Caucasian
Comments: This is one of two lines developed from this patient (see ATCC CRL 1594).
The line contains human papillomavirus type 18 (HPV-18) DNA sequences, and expresses HPV-18 RNA.
Propagation: ATCC complete growth medium: Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 3 times per week
Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
References: 22514: C-4 II細(xì)胞Auersperg N, Hawryluk AP. Chromosome observations on three epithelial-cell cultures derived from carcinomas of the human cervix. J. Natl. Cancer Inst. 28: 605-627, 1962. PubMed: 13863218
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
23180: Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217
26098: Auersperg N. Histogenetic behavior of tumors. I. Morphologic variation in vitro and in vivo of two related human carcinoma cell lines. J. Natl. Cancer Inst. 43: 151-173, 1969. PubMed: 5796380
26099: Auersperg N. Histogenetic behavior of tumors. II. Roles of cellular and environmental factors in the in vitro growth of carcinoma cells. J. Natl. Cancer Inst. 43: 175-190, 1969. PubMed: 5796382
26100: Auersperg N. Histogenetic behavior of tumors. 3. Possible relationships to patterns of glycolysis. J. Natl. Cancer Inst. 48: 1589-1596, 1972. PubMed: 5056250
26101: Lee HC, Auersperg N. Calcium in epithelial cell contraction. J. Cell Biol. 85: 325-336, 1980.C-4 II細(xì)胞 PubMed: 6768754
