LS513細(xì)胞
器官來源： 盲腸
生長狀態(tài)： 貼壁生長
運(yùn)輸方式： 凍存運(yùn)輸
年限： Dukes' type C
細(xì)胞形態(tài)： 上皮樣
數(shù)量： 大量
ATCC Number： CRL-2134?
相關(guān)**： 大腸癌
是否是腫瘤細(xì)胞： 1
物種來源： 人
Designations: LS513
Depositors: L Suardet
LS513細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: cecum
Tumor Stage: Dukes' type C
Disease: colorectal carcinoma
Cellular Products: transforming growth factor beta 1 (TGF beta-1, 83 pg per 10 exp6 cells per 24 hours)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. LS513細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Oncogene: p53 + (wild type)
Antigen Expression: carcinoembryonic antigen (CEA); ICAM-1; HLA class I positive
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,13
D13S317: 9,10
D16S539: 12,13
D5S818: 9,11
D7S820: 8,11
THO1: 8
TPOX: 8
vWA: 16,17
Cytogenetic Analysis: LS513細(xì)胞Two stem lines could be distinguished. The main one was represented in 65% of the cells, with a modal number of 51,XY and 3 markers, M1 - der(1)t(1;15), M2 - der(2)t(2;3)der(3)t(2;3), M3, and a monosomy 15., The second stem line had a modal chromosome number of 52,XY and presented M2 and M3 plus an isochromosome for the long arm of chromosome 1 called M4., A trisomy 5,7, a tetrasomy 13, and a monosomy 2 and 3 were present in all of the cells analyzed; the line did not exhibit monosomy 15.
Age: 63 years
Gender: male
Ethnicity: Caucasian
Comments: LS513 is a colorectal carcinoma cell line isolated in 1985 from a primary tumor biopsy from a 63 year old Caucasian male patient diagnosed with a Dukes' C mucin secreting cecal tumor located at the Bauhin valve.
Approximately 50% of LS513 cells express surface carcinoembryonic antigen (CEA).
LS513 cells express the major histocompatibility (MHC) class I antigens HLA and beta 2 microglobulin.
MHC class II antigens (HLA-DR, DQ, and DP were not detected).
TGF beta-1 is inhibitory for proliferation of LS513 cells, whereas TGF beta-2 has no effect on the growth of these cells.
LS513 cells are 100-fold less sensitive to TGF beta-1 than the LS1034 (ATCC CRL-2158) cell line.
LS513 cells are multidrug resistant (MDR) and are tumorigenic in nude mice.
Colony forming efficiency was 30% in methylcellulose.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: LS513細(xì)胞A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Preservation: Freeze medium: 50% Culture Medium + 40% FBS + 10% DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 25 hrs
References: 23099: Suardet L, et al. Responsiveness of three newly established human colorectal cancer cell lines to transforming growth factors beta 1 and beta 2. Cancer Res. 52: 3705-3712, 1992. PubMed: 1617643
23339: Burmester JK, et al. Characterization of distinct functional domains of transforming growth factor beta. Proc. Natl. Acad. Sci. USA 90: 8628-8632, 1993. PubMed: 7690965
23418: Li CY, et al. Potential role of WAF1/Cip1/p21 as a mediator of TGF-beta cytoinhibitory effect. J. Biol. Chem. 270: 4971-4974, 1995. PubMed: 7890601
