EB-3細(xì)胞
年限： 3 years
細(xì)胞類型： B**細(xì)胞
數(shù)量： 大量
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
ATCC Number： CCL-85?
相關(guān)**： **瘤
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： **樣
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
Designations: EB-3 [EB3]
EB-3細(xì)胞Depositors: W Henle
Biosafety Level: 2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Resistance: partially resistant to polioviruses and vesicular stomatitis virus
Antigen Expression: HLA A3, Aw32, Cw2
DNA Profile (STR): EB-3細(xì)胞Amelogenin: X,Y
CSF1PO: 12,15
D13S317: 12,14
D16S539: 10,12
D5S818: 9,10
D7S820: 11
THO1: 7
TPOX: 6,9
vWA: 17,19
Isoenzymes: G6PD, A
Age: 3 years
Gender: male
Ethnicity: Black
Comments: EBNA positive.
Propagation: EB-3細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively cultures can be established by centrifugation with subsequent resuspension at 3 X 10 exp5 viable cells/ml.
Preservation: culture medium, 90%; DMSO, 10%
References: 21415: Henle G, et al. Relation of Burkitt's tumor-associated herpes-ytpe virus to infectious mononucleosis. Proc. Natl. Acad. Sci. USA 59: 94-101, 1968. PubMed: 5242134
22143: Epstein MA, Barr YM. Cultivation in vitro of human lymphoblasts from Burkitt's malignant lymphoma. Lancet 1: 252-253, 1964. PubMed: 14090852
26251: . . Trans. N.Y. Acad. Sci. 29: 71, 1966.
26252: . . Wistar Inst. Symp. EB-3細(xì)胞Monogr. 4: 59, 1965.
