EB2細(xì)胞
細(xì)胞形態(tài)： **樣
年限： 7 years juvenile
運(yùn)輸方式： 凍存運(yùn)輸
ATCC Number： HTB-61?
相關(guān)**： **瘤
細(xì)胞類型： B**細(xì)胞
數(shù)量： 大量
器官來源： **結(jié)
是否是腫瘤細(xì)胞： 1
物種來源： 人
生長狀態(tài)： 懸浮生長
EB2細(xì)胞Designations: EB2 [EB-2]
Depositors: G Trempe, LJ Old
Biosafety Level: 2 [Cells Contain Eptein-Barr Virus ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Organ: lymph node
Disease: Burkitt's lymphoma
Derived from metastatic site: ovary
Cell Type: B lymphocyte;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. EB2細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1963
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,12
D13S317: 11,13
D16S539: 8,10
D5S818: 8,12
D7S820: 10,12
THO1: 9,9.3
TPOX: 8,9
vWA: 14,16
Cytogenetic Analysis: EB2細(xì)胞This is a hypertriploid human cell line with the modal chromosome number of 80, occurring in 30% of cells and polyploidy at 4.1%. Most cells had a chromosome number ranging between 79 and 85. Seven marker chromosomes were common to all cells, including der(14)t(8;14) (q22;q24) (two copies), i(5p), der(6)t(3;6) (p21.1;p21.1), der(11)t(7;11) (q11.23;q23.1) and two others. Four other marker chromosomes occurred only once among 11 metaphases analyzed. Most cells had four normal X chromosomes. No Y chromosomes were found in QM preparation. Some cells had five copies of N1 and/or N3.
Isoenzymes: ES-D, 1
G6PD, B
GLO-I, 2
PGM1, 1
Age: 7 years juvenile
Gender: female
Ethnicity: Black
Comments: The established EB2 line is similar, in general, to EB1 but differs in that it is composed of larger and less uniform cells which grow more rapidly than EB1. EB2細(xì)胞Furthermore, the EB2 cells regularly form clumps, have more cytoplasm, a less rounded nucleus and microvilli. Electron microscopic studies reveal viral particles (Epstein-Barr virus), lipid bodies, vacuoles, poorly developed mitochondria, frequent microvilli and an unusual layered structure projecting from the nucleus (a finding also reported for EB1 cells).The line is considered more primitive in differentiation than EB1.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 4 X 10(5) cells/ml and maintain at between 4 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Culture medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
References: 22722: . . Br. J. Cancer 19: 108-115, 1965.
23216: Giraldo G, et al. Kaposi's sarcoma: a new model in the search for viruses associated with human malignancies. J. Natl. Cancer Inst. 49: 1495-1507, 1972. PubMed: 4346014
26323: . . J. Exp. Med. 121: 761-770, 1965.
