SK-MEL-3細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
器官來(lái)源： 皮膚
細(xì)胞形態(tài)： 成纖維樣
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
年限： 42 years
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
ATCC Number： HTB-69?
相關(guān)**： 惡性黑色素瘤
Designations: SK-MEL-3
Depositors: G Trempe, LJ Old
SK-MEL-3細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: malignant melanoma
Derived from metastatic site: lymph node
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) SK-MEL-3細(xì)胞Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Tumorigenic: Yes
Antigen Expression: Blood Type O; Rh+
DNA Profile (STR): Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13
D16S539: 11
D5S818: 11
D7S820: 8,10
THO1: 6
TPOX: 8
vWA: 14,18
Cytogenetic Analysis: (P13) hypotetraploid to hypertetraploid with abnormalities including dicentrics, pulverizations, secondary constrictions and minutes
Isoenzymes: AK-1, 1
ES-D, 1
SK-MEL-3細(xì)胞G6PD, B
GLO-I, 1-2
PGM1, 1-2
PGM3, 1
Age: 42 years
Gender: female
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified , Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 1. Remove and discard culture medium.2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor.3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 5. Add appropriate aliquots of the cell suspension to new culture vessels. Subcultivation Ratio: 1:3 to 1:56. Incubate cultures at 37?C.
Subcultivation Ratio: SK-MEL-3細(xì)胞A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Culture medium, 95%; DMSO, 5%
References: 21869: . Human tumor cells in vitro. New York: Plenum Press; 1975.
22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
32291: Guldberg P, et al. Disruption of the MMAC1/PTEN gene by deletion or mutation is a frequent event in malignant melanoma. Cancer Res. 57: 3660-3663, 1997. PubMed: 9288767
