COLO 201細(xì)胞

是否是腫瘤細(xì)胞： 1

物種來源： 人

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CCL-224?

相關(guān)**： 大腸癌

器官來源： 結(jié)腸

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)，少量貼壁

細(xì)胞形態(tài)： 其他

年限： Dukes' type D

數(shù)量： 大量

COLO 201細(xì)胞Designations: COLO 201

Depositors: GE Moore

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, with some loosely adherent cells

Organism: Homo sapiens

Morphology: bipolar, slightly refractile, fibroblast-like cell

Source: Organ: colon

Tumor Stage: Dukes' type D

Disease: colorectal adenocarcinoma

Derived from metastatic site: ascites

Permits/Forms: COLO 201細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Oncogene: myc +; ras + ; myb + ; fos +; sis +; p53 +; abl -; ros -; src -

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,12

D13S317: 10,12

D16S539: 12,13

D5S818: 10,13

D7S820: 9,10

THO1: 8,9

TPOX: 11

vWA: 15

Cytogenetic Analysis: The stemline chromosome number is hypertriploid and 10-11 marker chromosomes (M1, M2, M4, M5, M6 and 5 others) were common in monosomic, disomic and trisomic condition although most of these markers were monosomic.

COLO 201細(xì)胞Isoenzymes: ES-D, 1-2

G6PD, B

PEP-D, 1

PGD, A

PGM1, 1-2

PGM3, 1-2

Age: 70 years

Gender: male

Ethnicity: Caucasian

Comments: The line is negative for CSAp (CSAp-) and CEA.

It was derived from tissue from the same patient as COLO 205 (ATCC CCL-222).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a 0.25% trypsin, 0.53 mM EDTA solution. Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. COLO 201細(xì)胞Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended

Medium Renewal: 1 to 2 times per week

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

derived from same individual:ATCC CCL-222

derived from same individual:ATCC CCL-224

References: 1046: Semple TU, et al. Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model. Cancer Res. 38: 1345-1355, 1978. PubMed: 565251

22861: Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874