NCI-H211細(xì)胞

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

運(yùn)輸方式： 凍存運(yùn)輸

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

數(shù)量： 大量

器官來(lái)源： 肺

ATCC Number： CRL-5824?

相關(guān)**： 小細(xì)胞肺癌

年限： stage E

Designations: NCI-H211 [H211]

Depositors: AF Gazdar, JD Minna

NCI-H211細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology:

Source: Organ: lung

Tumor Stage: stage E

Disease: carcinoma; small cell lung cancer

Derived from metastatic site: bone marrow

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. NCI-H211細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 9

D16S539: 13

D5S818: 11

D7S820: 10,12

THO1: 6

TPOX: 11

vWA: 14,18

NCI-H211細(xì)胞Cytogenetic Analysis: iso(3p), t(3;4)(pter-q12), t(3;11)(qter-p25)

Age: 50 years

Gender: female

Ethnicity: Caucasian, White

Comments: The line was established in January 1980.

The patient received prior chemotherapy and radiation therapy.

Fast growing.

The patient was a smoker.

35 pack years.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)

Cultures can be maintained by addition of fresh medium.

Cells grow as floating aggregates of round cell clusters.

Preservation: NCI-H211細(xì)胞Culture medium, 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.