Hs 688(A).T細胞
運輸方式： 凍存運輸
器官來源： 皮膚
ATCC Number： CRL-7425?
相關(guān)**： 黑色素瘤
細胞形態(tài)： 成纖維樣
數(shù)量： 大量
是否是腫瘤細胞： 1
物種來源： 人
年限： 69 years
生長狀態(tài)： 貼壁生長
Designations: Hs 688(A).T
Hs 688(A).T細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: melanoma
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Cytogenetic Analysis: modal number = 46; range = 45 to 47
Age: 69 years
Gender: male
Ethnicity: Caucasian
Comments: Metastasis to inguinal lymph node also was cultured (see ATCC CRL-7426).
Hs 688(A).T細胞Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Please see the NBL Repository description.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: Hs 688(A).T細胞A subcultivation ratio of 1:2 to 1:3 is recommended
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
derived from same individual:Hs 688(A).T細胞ATCC CRL-7426
