Hs 766T細(xì)胞
器官來源： 胰腺
細(xì)胞形態(tài)： 上皮樣
ATCC Number： HTB-134?
相關(guān)**： 腫瘤
年限： 46 years
生長狀態(tài)： 貼壁生長
數(shù)量： 大量
是否是腫瘤細(xì)胞： 1
物種來源： 人
運(yùn)輸方式： 凍存運(yùn)輸
Designations: Hs 766T
Depositors: RB Owens
Hs 766T細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: pancreas
Disease: carcinoma
Derived from metastatic site: lymph node
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host (Roche Transfection Reagents)
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 12
D13S317: 8,11
D16S539: 9,12
Hs 766T細(xì)胞D5S818: 11
D7S820: 10
THO1: 6,9.3
TPOX: 8
vWA: 18
Cytogenetic Analysis: (P11) hyperdiploid with mode = 60; banded markers; lacking Y chromosomes
Isoenzymes: AK-1, 1
ES-D, 1-2
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1-2
PGM3, 1
Age: 46 years
Gender: male
Ethnicity: Caucasian
Comments: The Hs 766T line was isolated by R. Owens et al. at the Naval Biosciences Laboratory in 1973 from a pancreatic carcinoma metastatic to a lymph node.
The epithelial nature of the cell strain selected was confirmed by electron microscopy as microvilli and desmosomes were observed.
Hs 766T細(xì)胞Tests conducted at an early passage indicated that the cells exhibited many properties observed only in tumor lines. The karyotype was abnormal and the cells formed colonies in Methocel and on contact inhibited monolayers.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 22534: Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412
22543: Smith HS. Hs 766T細(xì)胞In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. PubMed: 283258
23079: Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903
26328: . Prevention and detection of cancer. vol. 2New York: Marcel Dekker; 1978.
